This comprehensive WHO guideline provides global, evidence-informed recommendations on routine antenatal care. The guidance aims to capture the complex nature of the issues surrounding ANC health care practices and delivery, and to prioritize person-centred health and well-being, not only the prevention of death and morbidity, in accordance with a human rights-based approach. This guideline is relevant to all pregnant women and adolescent girls receiving ANC in any healthcare or community setting and their unborn fetuses and newborns.